Knives of High Silica Content Glass for Thin-Sectioning

نویسندگان

  • Mary Ann Gavin
  • Bolivar J. Lloyd
چکیده

The use of glass knives in cutting sections for electron microscopy has been a standard procedure for several years. During this period various modifications in methods of scoring and breaking knives have been recommended, as well as types of glass which can be used (1, 2). Knives of high silica content glass were first suggested by Eaves and Flewett (1953) who reported that such knives retained a sharp edge longer and gave generally better sections than soft glass (3). At least two kinds of high silica glass are commercially available in this country, pyrex brand and vycor brand plate? Both types of glass were used to section muscle, lens capsule and epithelium, lens fibers, Limulus eye, and residues obtained from lens fractionation. All tissues were fixed with buffered, I per cent osmium tetroxide and embedded in a mixture of butyland methyl-methacrylates in the ratios of either 9:1 or 4:1. The plastic was polymerized at 65°C. either in a nitrogen atmosphere (4) or in a conventional incubator. Since vycor brand glass proved to be superior to pyrex in sectioning qualities, i.e. it produced fewer striations, and had a longer lasting cutting edge, the following discussion concerns this type of glass only. Vycor glass can be obtained in 6 inch x 6 inch sheets, 1~ inch thick, with polished edges. In the laboratory, such a plate is cut into three strips, each two inches wide. These strips are then scored and easily broken according to the method of Cameron (2). However, in order to obtain a less pronounced curvature of the edge, the knives are broken between two glazier's pliers rather than with a heated glass rod.

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عنوان ژورنال:
  • The Journal of Biophysical and Biochemical Cytology

دوره 5  شماره 

صفحات  -

تاریخ انتشار 1959